Interoperability between Cooperative Design Modeller and a CAD System: Software Integration versus Data Exchange

The data exchange between Computer-Aided Design (CAD) systems is a crucial issue in concurrent engineering and collaborative design. The paper presents research works and techniques dealing with the interoperability of a Cooperative Design Modeller (CoDeMo), aiming at the integration of product lifecycle knowledge, and a commercial CAD system (CATIA V5). Two kinds of approaches are implemented in the considered case of CAD interoperability for exchanging geometric data, respectively: one is based on a traditional static interface, in which STEP AP203 standard is used; the other is based on a dynamic interface, in which Application Programming Interfaces (API) of the targeted CAD system is adopted. Both approaches should enhance the communication, exchange and sharing of product data between CAD systems for improving concurrent engineering. A comparison between these two approaches is made to show their particular advantages and disadvantages. The development of a translator between the both CAD systems based on each approach has been carried out and evaluated on an assembly case

Interoperability between Cooperative Design Modeller and a CAD System: Software Integration versus Data Exchange

International audienceThe data exchange between Computer-Aided Design (CAD) systems is a crucial issue in concurrent engineering and collaborative design. The paper presents research works and techniques dealing with the interoperability of a Cooperative Design Modeller (CoDeMo), aiming at the integration of product lifecycle knowledge, and a commercial CAD system (CATIA V5). Two kinds of approaches are implemented in the considered case of CAD interoperability for exchanging geometric data, respectively: one is based on a traditional static interface, in which STEP AP203 standard is used; the other is based on a dynamic interface, in which Application Programming Interfaces (API) of the targeted CAD system is adopted. Both approaches should enhance the communication, exchange and sharing of product data between CAD systems for improving concurrent engineering. A comparison between these two approaches is made to show their particular advantages and disadvantages. The development of a translator between the both CAD systems based on each approach has been carried out and evaluated on an assembly case

Interoperability between Cooperative Design Modeller and a CAD System: Software Integration versus Data Exchange

The data exchange between Computer-Aided Design (CAD) systems is a crucial issue in concurrent engineering and collaborative design. The paper presents research works and techniques dealing with the interoperability of a Cooperative Design Modeller (CoDeMo), aiming at the integration of product lifecycle knowledge, and a commercial CAD system (CATIA V5). Two kinds of approaches are implemented in the considered case of CAD interoperability for exchanging geometric data, respectively: one is based on a traditional static interface, in which STEP AP203 standard is used; the other is based on a dynamic interface, in which Application Programming Interfaces (API) of the targeted CAD system is adopted. Both approaches should enhance the communication, exchange and sharing of product data between CAD systems for improving concurrent engineering. A comparison between these two approaches is made to show their particular advantages and disadvantages. The development of a translator between the both CAD systems based on each approach has been carried out and evaluated on an assembly case

Towards Integration of CAx Systems and a Mul ti ple-View Prod uct Mod el ler in Mechanical Design

This paper deals with the development of an integration framework and its implementation for the connexion of CAx systems and multiple-view product modelling. The integration framework is presented regarding its conceptual level and the implementation level is described currently with the connexion of a functional modeller, a multiple-view product modeller, an optimisation module and a CAD system. The integration between the multiple-view product modeller and CATIA V5 based on the STEP standard is described in detail. Finally, the presented works are discussed and future research developments are suggested

Transcriptome of Small Regulatory RNAs in the Development of the Zoonotic Parasite Trichinella spiralis

BACKGROUND: Trichinella spiralis is a parasite with unique features. It is a multicellular organism but with an intracellular parasitization and development stage. T. spiralis is the helminthic pathogen that causes zoonotic trichinellosis and afflicts more than 10 million people worldwide, whereas the parasite's biology, especially the developmental regulation is largely unknown. In other organisms, small non-coding RNAs, such as microRNAs (miRNA) and small interfering RNAs (siRNA) execute post-transcriptional regulation by translational repression or mRNA degradation, and a large number of miRNAs have been identified in diverse species. In T. spiralis, the profile of small non-coding RNAs and their function remains poorly understood. METHODOLOGY AND PRINCIPAL FINDINGS: Here, the transcriptional profiles of miRNA and siRNA in three developmental stages of T. spiralis in the rat host were investigated, and compared by high-throughput cDNA sequencing technique ("RNA-seq"). 5,443,641 unique sequence tags were obtained. Of these, 21 represented conserved miRNAs related to 13 previously identified metazoan miRNA families and 213 were novel miRNAs so far unique to T. spiralis. Some of these miRNAs exhibited stage-specific expression. Expression of miRNAs was confirmed in three stages of the life cycle by qRT-PCR and northern blot analysis. In addition, endogenous siRNAs (endo-siRNAs) were found mainly derived from natural antisense transcripts (NAT) and transposable elements (TE) in the parasite. CONCLUSIONS AND SIGNIFICANCE: We provide evidence for the presence of miRNAs and endo-siRNAs in T. spiralis. The miRNAs accounted for the major proportion of the small regulatory RNA population of T. spiralis, while fewer endogenous siRNAs were found. The finding of stage-specific expression patterns of the miRNAs in different developmental stages of T. spiralis suggests that miRNAs may play important roles in parasite development. Our data provide a basis for further understanding of the molecular regulation and functional evolution of miRNAs in parasitic nematodes

Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity

Background: ORFV attenuated live vaccines have been the main prophylactic measure against contagious ecthyma in sheep and goats in the last decades, which play an important role in preventing the outbreak of the disease. However, the available vaccines do not induce lasting immunity in sheep and goats. On the other hand, variation in the terminal genome of Orf virus vaccine strains during cell culture adaptation may affect the efficacy of a vaccine. Currently, there are no more effective antiviral treatments available for contagious ecthyma. Results: We constructed three eukaryotic expression vectors pcDNA3.1-ORFV011, pcDNA3.1-ORFV059 and pcDNA3.1-ORFV011/ORFV059 and tested their immunogenicity in mouse model. High level expression of the recombinant proteins ORFV011, ORFV059 and ORFV011/ORFV059 was confirmed by western blotting analysis and indirect fluorescence antibody (IFA) tests. The ORFV-specific antibody titers and serum IgG1/IgG2a titers, the proliferation of lymphocytes and ORFV-specific cytokines (IL-2, IL-4, IL-6, IFN-gamma, and TNF-alpha) were examined to evaluate the immune responses of the vaccinated mice. We found that mice inoculated with pcDNA3.1-ORFV 011/ORFV059 had significantly stronger immunological responses than those inoculated with pcDNA3.1-ORFV011, pcDNA3.1-ORFV059, or pcDNA3.1-ORFV011 plus pcDNA3.1-ORFV059. Compared to other vaccine plasmids immunized groups, pcDNA3.1-ORFV011/ORFV059 immunized group enhances immunogenicity. Conclusions: We concluded that DNA vaccine pcDNA3.1-ORFV011/ORFV059 expressing ORFV011 and ORFV059 chemeric-proteins can significantly improve the potency of DNA vaccination and could be served as more effective and safe approach for new vaccines against ORFV.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000304650500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701VirologySCI(E)3ARTICLEnull